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Operations During COVID-19

Microscopy: Nikon LV100ND Optical Microscope

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Overview

The Nikon LV100ND Optical Microscope features:

  • Both top and bottom lighting using LED white light with adjustable intensity.
  • 5X, 10X, 20X, 50X, and 100X Plan Fluor high numerical aperture lenses.
  • 10X Ultra Wide Field
  • Brightfield and darkfield for all five lenses.  Brightfield directs the light straight down the center
  • of view, while darkfield lights only from an oblique angle to highlight non-flat surfaces.
  • Selectable Nomarski Differential Interference Contrast (for top down lighting only).  Nomarski DIC allows you to see subtle details, alter the colors of different surfaces, and it provides a 3D look to the image applying dark shadows on one side of 3D feature and bright highlights on opposite side.
  • DIC prism slider
  • Rotatable Polarizer Slider for Episcopic illumination.
  • Analyzer Slider for Episcopic illumination.
  • Simple Polarizer for Diascopic illumination.
  • A 6" x 4" XY stage with glass plate.
  • An Abbe Condenser for concentrating and focusing lighting from below the stage.
  • A high definition (18 Megapixel) DS-Ri2 Nikon camera.
  • A "smart turret" which communicates which objective is being used to the computer.
  • Nikon NIS-Elements D Software with wide range of advanced features.
  • Camera image is carefully calibrated, so software can take accurate point to point distance measurements.   
  • Metadata from image can be stored along with image.

Contact Information

Both the Flexible and Nanopatterning Cleanrooms have a Nikon LV100ND.

Flexible Cleanroom: Tom Carver
650 723-1861 

Nanopatterning Cleanroom: nano-snpcleanroom-staff@lists.stanford.edu

Getting Started and Training Information

In order to become a qualified user of the Nikon optical microscope, you need to follow each of these steps in the order as listed here:

  1. Complete the process to become a lab member of SNSF and follow the instructions to activate a Badger account.
  2. Complete Axess STARS EHS-2470-WEB: COVID-19 Hygiene Best Practices.
  3. Flexible Cleanroom:
    1. Complete all requirements to become a qualified user for the Flexible Cleanroom.
    2. Arrange for training by contacting Tom Carver.
  4. Nanopatterning Cleanroom:
    1. Complete all requirements to become a qualified user for the Nanopatterning Cleanroom.
    2. No further training is required. If you require assistance with the tool, please contact nano-snpcleanroom-staff@lists.stanford.edu.