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Leica UC Enuity Ultramicrotome

Used to make TEM sections

Prerequisites

  • Users must have an active Nemo account
  • Must complete Moore 184 safety orientation
  • Read and understand the SOP
  • Submit training request in Nemo

Overview

The Leica UC Enuity ultramicrotome is used to make thin sections of material for transmission electron microscopy (TEM).  TEM sections can be made at room temperature or at cryogenic temperatures.  The system can produce 30 nm thin TEM sections, and is more suitable for soft materials, such as polymers, soft metals, biological samples, and composites.  

  • Room temperature sectioning
  • Cryo-sectioning with LN2
  • Site specific sectioning with 3D microCT image data
  • Array tomography sectioning
  • Cross section polishing

 

 

SEM Image of Polished Block Face Created Using Ultramicrotome Showing Biopolymer Bridging in Engineered Composite
HRTEM Image of Nickel Sample Demonstrating Atomic Resolution Can Be Achieved With Microtome Sectioning of Soft Metals

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System Specifications

Ultramicrotomy

Goal

  • (S)TEM,
  • AFM

Sample Prep

  • Ultramicrotomy,
  • Cryo

Sample Type

  • Biological,
  • Polymer,
  • Soft Material,
  • Metals