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Operations During COVID-19

Microscopy: Keyence VK-X250/260K 3D Laser Scanning Confocal Microscope

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The Keyence VK-X Series 3D Laser Scanning Confocal Microscope provides non-contact, nanometer-level profile, roughness, and film thickness data on any material. The lateral resolution of the microscope is 120 nm using the 408 nm Violet laser light. The microscope scans the surface using a 16-bit photomultiplier to receive the reflected laser light. The instrument is able to provide highly accurate 3D measurement data over any shape of materials and steep angles (up to 88 Degree angle of detection). High-resolution optical images can be acquired with resolutions up to 21.6 million pixels through 3CCD pixel shifting technology. High dynamic range (16-bit resolution color gradation) function automatically recognizes bright and dark areas as well as low contrast areas.

Getting Started and Training Information

In order to become a qualified user of the Keyence Laser Microscope, you need to follow each of these steps in the order as listed here:

  1. Complete the process to become a lab member of SNSF and follow the instructions to activate a Badger account.
  2. Complete Axess STARS EHS-2470-WEB: COVID-19 Hygiene Best Practices.
  3. Complete the process to become a Nanopatterning Cleanroom user.
  4. Review the Resources section below for an introduction to the Keyence 3D Confocal Microscope and how to operate it.
  5. Pass (80%) a quiz that reviews how to operate the Keyence Microscope: Keyence 3D Laser Confocal Microscope Review Quiz.
  6. Complete the Keyence Microscope New User Intake Form (second part of the quiz above).
  7. Record your name and email down on the Keyence Microscope Training Calendar (sent to you by email after completing the New User Intake Form).
  8. Staff will reach out to schedule a one-on-one remote qualification session. You will be present in the cleanroom by the tool while staff is remoted into the Keyence computer and on Zoom. During the qualification session, you will operate the tool while a staff member observes and ensures you know how to operate the tool. You are welcome to bring your own sample(s) to the session if you would like to discuss further regarding your sample(s) after completing the qualification components. Any features that were not discussed in the SOP or videos may be taught during the qualification session depending on how you plan to use the tool and what your samples may look like.